Dataset Information

Centriolin interacts with HectD1 in a cell cycle dependent manner.

ABSTRACT:

Objective

The centrosome is universally recognized as the microtubule organizing center of animal cells, but emerging evidence suggests that it has other important functions including primary cilia formation, DNA damage checkpoints, and cell cycle progression. Despite this, the role of individual components of the centrosome remains unclear. Previous studies suggest that one component, centriolin, has an important function in cytokinesis and cell cycle progression, although its exact role in these processes is not known. To determine how centriolin influences the progression through the cell cycle, we sought to identify interacting partners that may be involved in regulating its function.

Results

This study provides evidence that the ubiquitin E3 ligase HectD1 binds to centriolin and that this association likely accounts for our observation that HectD1 co-localizes with centriolin at the centrosome during mitosis. In addition to its centrosomal localization, we also show that the expression of HectD1 fluctuates throughout the cell cycle, with the highest levels during mitosis, coinciding with a marked reduction in centriolin expression. We propose that the interaction between HectD1 and centriolin may be necessary for normal cell cycle progression and we speculate that this function may involve HectD1-mediated degradation of centriolin.

SUBMITTER: Salas J

PROVIDER: S-EPMC10731774 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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Publications

Centriolin interacts with HectD1 in a cell cycle dependent manner.

Salas Jesus J Garcia Alexander A Zora Vancy V Dornbush Sean S Mousa-Ibrahim Fady F Fogg Hanna H Gromley Zeynep Z Gromley Adam A

BMC research notes 20231219 1

<h4>Objective</h4>The centrosome is universally recognized as the microtubule organizing center of animal cells, but emerging evidence suggests that it has other important functions including primary cilia formation, DNA damage checkpoints, and cell cycle progression. Despite this, the role of individual components of the centrosome remains unclear. Previous studies suggest that one component, centriolin, has an important function in cytokinesis and cell cycle progression, although its exact rol ...[more]

PMID: 38115153

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Dataset Information

Centriolin interacts with HectD1 in a cell cycle dependent manner.

Objective

Results

Publications

Centriolin interacts with HectD1 in a cell cycle dependent manner.

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