Characterization of an iPSC-based barrier model for blood-brain barrier investigations using the SBAD0201 stem cell line.

ABSTRACT:

Background

Blood-brain barrier (BBB) models based on primary murine, bovine, and porcine brain capillary endothelial cell cultures have long been regarded as robust models with appropriate properties to examine the functional transport of small molecules. However, species differences sometimes complicate translating results from these models to human settings. During the last decade, brain capillary endothelial-like cells (BCECs) have been generated from stem cell sources to model the human BBB in vitro. The aim of the present study was to establish and characterize a human BBB model using human induced pluripotent stem cell (hiPSC)-derived BCECs from the hIPSC line SBAD0201.

Methods

The model was evaluated using transcriptomics, proteomics, immunocytochemistry, transendothe

SUBMITTER: Ozgur B 

PROVIDER: S-EPMC10731806 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature