Establishment and validation of a callus tissue transformation system for German chamomile (Matricaria chamomilla L.).

ABSTRACT:

Background

German chamomile (Matricaria chamomilla L.) is an important medicinal plant, and the essential oils in the flowers have various biological activities. Genetic transformation systems are important for plant quality improvement and molecular research. To the best of our knowledge, a genetic transformation system has not yet been reported for German chamomile.

Results

In this study, we developed Agrobacterium-mediated transformation protocols for German chamomile callus tissues. This involved optimizing key parameters, such as hygromycin and cefotaxime concentrations, bacterial density, and infection and co-culture durations. We also performed gas chromatography-mass spectrometry analysis to identify volatile compounds in non-transgenic and transgenic callus and hair

SUBMITTER: Tai Y 

PROVIDER: S-EPMC10731808 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature