Simian immunodeficiency virus and storage buffer: Field-friendly preservation methods for RNA viral detection in primate feces.

ABSTRACT:

Importance

Simian immunodeficiency virus (SIV), which originated in African monkeys, crossed the species barrier into humans and ultimately gave rise to HIV and the global HIV/AIDS epidemic. While SIV infects over 40 primate species in sub-Saharan Africa, testing for RNA viruses in wild primate populations can be challenging. Optimizing field-friendly methods for assessing viral presence/abundance in non-invasively collected biological samples facilitates the study of viruses, including potentially zoonotic viruses, in wild primate populations. This study compares SIV RNA preservation and recovery from non-human primate feces stored in four different buffers. Our results will inform future fieldwork and facilitate improved approaches to characterizing prevalence, shedding, and tran

SUBMITTER: Wilde THC 

PROVIDER: S-EPMC10732032 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature