Single-cell quantitative bioimaging of <i>P. berghei</i> liver stage translation.

ABSTRACT:

Importance

Plasmodium parasites cause malaria in humans. New multistage active antimalarial drugs are needed, and a promising class of drugs targets the core cellular process of translation, which has many potential molecular targets. During the obligate liver stage, Plasmodium parasites grow in metabolically active hepatocytes, making it challenging to study core cellular processes common to both host cells and parasites, as the signal from the host typically overwhelms that of the parasite. Here, we present and validate a flexible assay to quantify Plasmodium liver stage translation using a technique to fluorescently label the newly synthesized proteins of both host and parasite followed by computational separation of their respective nascent proteomes in con

SUBMITTER: McLellan JL 

PROVIDER: S-EPMC10732057 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature