Dataset Information

Phylogenetic diversity and functional potential of large and cell-associated viruses in the Bay of Bengal.

ABSTRACT:

Importance

The BoB, the world's largest bay, is of significant economic importance to surrounding countries, particularly Bangladesh, which heavily relies on its coastal resources. Concurrently, the BoB holds substantial ecological relevance due to the region's high vulnerability to climate change-induced impacts. Yet, our understanding of the BoB's microbiome in relation to marine food web and biogeochemical cycling remains limited. Particularly, there are little or no data on the viral diversity and host association in the BoB. We examined the viral community in two distinct BoB coastal regions to reveal a multitude of viral species interacting with a wide range of microbial hosts, some of which play key roles in coastal biogeochemical cycling or potential pathogens. Furthermore, we demonstrate that the BoB coast harbors a diverse community of large and giant viruses, underscoring the importance of investigating understudied environments to discover novel viral lineages with complex metabolic capacities.

SUBMITTER: Minch B

PROVIDER: S-EPMC10732071 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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Phylogenetic diversity and functional potential of large and cell-associated viruses in the Bay of Bengal.

Minch Benjamin B Akter Salma S Weinheimer Alaina A Rahman M Shaminur MS Parvez Md Anowar Khasru MAK Rezwana Rahman Sabita S Ahmed Md Firoz MF Moniruzzaman Mohammad M

mSphere 20231030 6

<h4>Importance</h4>The BoB, the world's largest bay, is of significant economic importance to surrounding countries, particularly Bangladesh, which heavily relies on its coastal resources. Concurrently, the BoB holds substantial ecological relevance due to the region's high vulnerability to climate change-induced impacts. Yet, our understanding of the BoB's microbiome in relation to marine food web and biogeochemical cycling remains limited. Particularly, there are little or no data on the viral ...[more]

PMID: 37902318

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Project description:Rhizobacteria associated with cultivated crops are known to stimulate plant growth through various indirect or direct mechanisms. In recent years, the host list of plant growth promotion/promoting rhizobacteria has expanded to include bean, barley, cotton, maize, rice, vegetables, peanut, rice, wheat, and several plantation crops. However, interaction of rhizobacteria with tea plants of organic and conventional tea gardens is poorly understood. In the present study, rhizobacterial species associated with tea rhizosphere were isolated from 14 tea gardens located in North Bengal, India. In total, 16 rhizobacterial isolates isolated from collected soil samples were assessed for antagonistic and plant growth promotion/promoting activity under laboratory conditions. Molecular characterization based on sequencing of 16S rRNA gene revealed dominance of Bacillus with five species followed by Pseudomonas with two species. Interestingly, only one isolate was affiliated with actinobacteria, i.e., Microbacterium barkeri. Out of 16 isolates, isolates Bacillus subtilis OKAKP01, B. subtilis BNLG01, B. paramycoides BOK01, M. barkeri BPATH02, and Stenotrophomonas maltophilia BSEY01 showed highest growth inhibition against Fusarium solani (68.2 to 72.8%), Pseudopestalotiopsis theae (71.1 to 85.6%), and Exobasidium vexans (67.4 to 78.3%) causing respective Fusarium dieback, gray blight, and blister blight diseases in tea crop. Further, these five isolates also possessed significantly greater antifungal (siderophore producer, protease, chitinase, and cellulase activity) and plant growth promotion/promoting (indole-3-acetic acid production, ACC deaminase, ammonia, and phosphate solubilization) traits over other eleven rhizobacterial isolates. Therefore, these five isolates of rhizobacteria were chosen for their plant growth promotion/promoting activity on tea plants in nursery conditions. Results from nursery experiments revealed that these five rhizobacteria significantly improved growth rates of tea plants compared with the control. Therefore, this study suggests that these rhizobacteria could be used to formulate biopesticides and biofertilizers, which could be applied to sustainable tea cultivation to improve crop health and reduce disease attack.

Project description:BackgroundThis study was carried out to classify the diversity of the deep marine psychrotolerant actinomycetes sp. nov., in the Bay of Bengal and exploit the production of cold-active industrial and pharmaceutical biomolecules.Objective1) Characterization, optimum the growth conditions and classify the diversity of the novel isolated deep marine psychrotolerant actinomycetes sp from the Bay-of-Bengal. 2) Screening for industrially important biocatalysts and determine the antimicrobial activities against the five dreadful pathogens. 3) The differential expression profiling of the candidate genes to regulate the biosynthesis of selected enzymes.MethodsThe cold-adapted actinomycetes were isolated from the deep marine water collections at 1200 mts below the surface in Bay-of-Bengal. The phenotypic and genotypic characterizations have been carried out to understand the persistent diversity of this novel marine psychrotolerant actinomycetes species. The production of cold-active enzymes, such as amylase, cellulase, lipase, pectinase, and L-asparaginase, were screened and the expression profiling genes were determined by using qRT PCR. The antibacterial and antifungal activities have also been investigated.ResultsA total number of 37 novel actinomycetes were isolated and the phenotypic and genotypic characterizations identified the genus, dominated by Streptomyces (17 distinct sub-groups) as the major group, followed by Micromonospora, Actinopolyspora, Actinosynnema, Streptoverticillium, Saccharopolyspora, Nocardiopsis, and Nocardia. The optimum growth and abundant mycelium formation are observed at 15°C to 20°C and also capability for thriving at 4°C. All the isolates exhibited a significant role in the production of biocatalysts, and the antagonistic activities were also noted against five major selected pathogens.ConclusionThe Streptomyces from the Bay-of-Bengal have high biosynthetic potential and can serve as a good resource for the exploration of bioactive natural products.

Dataset Information

Phylogenetic diversity and functional potential of large and cell-associated viruses in the Bay of Bengal.

Importance

Publications

Phylogenetic diversity and functional potential of large and cell-associated viruses in the Bay of Bengal.

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