Oral Metformin Inhibits Choroidal Neovascularization by Modulating the Gut-Retina Axis.

ABSTRACT:

Purpose

Emerging data indicate that metformin may prevent the development of age-related macular degeneration (AMD). Whereas the underlying mechanisms of metformin's anti-aging properties remain undetermined, one proposed avenue is the gut microbiome. Using the laser-induced choroidal neovascularization (CNV) model, we investigate the effects of oral metformin on CNV, retinal pigment epithelium (RPE)/choroid transcriptome, and gut microbiota.

Methods

Specific pathogen free (SPF) male mice were treated via daily oral gavage of metformin 300 mg/kg or vehicle. Male mice were selected to minimize sex-specific differences to laser induction and response to metformin. Laser-induced CNV size and macrophage/microglial infiltration were assessed by isolectin and Iba1 immunostaining.

SUBMITTER: Zhang JY 

PROVIDER: S-EPMC10732090 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature