T3SS chaperone of the CesT family is required for secretion of the anti-sigma factor BtrA in Bordetella pertussis.

ABSTRACT: Bordetella pertussis is a Gram-negative, strictly human re-emerging respiratory pathogen and the causative agent of whooping cough. Similar to other Gram-negative pathogens, B. pertussis produces the type III secretion system, but its role in the pathogenesis of B. pertussis is enigmatic and yet to be elucidated. Here, we combined RNA-seq, LC-MS/MS, and co-immunoprecipitation techniques to identify and characterize the novel CesT family T3SS chaperone BP2265. We show that this chaperone specifically interacts with the secreted T3SS regulator BtrA and represents the first non-flagellar chaperone required for the secretion of an anti-sigma factor. In its absence, secretion but not production of BtrA and most T3SS substrates is severely impaired. It appears that the role

SUBMITTER: Drzmisek J 

PROVIDER: S-EPMC10732220 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature