CPMAS NMR platform for direct compositional analysis of mycobacterial cell-wall complexes and whole cells.

ABSTRACT: Tuberculosis and non-tuberculosis mycobacterial infections are rising each year and often result in chronic incurable disease. Important antibiotics target cell-wall biosynthesis, yet some mycobacteria are alarmingly resistant or tolerant to currently available antibiotics. This resistance is often attributed to assumed differences in composition of the complex cell wall of different mycobacterial strains and species. However, due to the highly crosslinked and insoluble nature of mycobacterial cell walls, direct comparative determinations of cell-wall composition pose a challenge to analysis through conventional biochemical analyses. We introduce an approach to directly observe the chemical composition of mycobacterial cell walls using solid-state NMR spectroscopy. ¹³C CPMAS spe

SUBMITTER: Liu X 

PROVIDER: S-EPMC10732466 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature