Benchmarking software tools for trimming adapters and merging next-generation sequencing data for ancient DNA.

ABSTRACT: Ancient DNA is highly degraded, resulting in very short sequences. Reads generated with modern high-throughput sequencing machines are generally longer than ancient DNA molecules, therefore the reads often contain some portion of the sequencing adaptors. It is crucial to remove those adaptors, as they can interfere with downstream analysis. Furthermore, overlapping portions when DNA has been read forward and backward (paired-end) can be merged to correct sequencing errors and improve read quality. Several tools have been developed for adapter trimming and read merging, however, no one has attempted to evaluate their accuracy and evaluate their potential impact on downstream analyses. Through the simulation of sequencing data, seven commonly used tools were analyzed in their ability to reco

SUBMITTER: Lien A 

PROVIDER: S-EPMC10733496 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature