Development of a quadruplex PCR amplicon next generation sequencing assay for detection and differentiation of Bartonella spp.

ABSTRACT: The genus Bartonella includes a group of species that are associated with a wide range of mammalian species, including human. It is challenging to detect all Bartonella species using a single molecular target due to its high genetic diversity. To solve this issue, we developed a quadruplex PCR amplicon sequencing assay using next-generation sequencing (NGS) technology for the detection and differentiation of Bartonella species. Our objective was to obtain the specific sequences of a minimum of two of the four target genes as confirmation of the identity of a particular Bartonella species using the assay. Four pairs of primers targeting specific regions on gltA, groEL, rpoB, and ssrA were evaluated for their capability of differentiating Bartonel

SUBMITTER: Bai Y 

PROVIDER: S-EPMC10733521 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature