Patient-derived glioblastoma organoids reflect tumor heterogeneity and treatment sensitivity.

ABSTRACT:

Background

Treatment resistance and tumor relapse are the primary causes of mortality in glioblastoma (GBM), with intratumoral heterogeneity playing a significant role. Patient-derived cancer organoids have emerged as a promising model capable of recapitulating tumor heterogeneity. Our objective was to develop patient-derived GBM organoids (PGO) to investigate treatment response and resistance.

Methods

GBM samples were used to generate PGOs and analyzed using whole-exome sequencing (WES) and single-cell karyotype sequencing. PGOs were subjected to temozolomide (TMZ) to assess viability. Bulk RNA sequencing was performed before and after TMZ.

Results

WES analysis on individual PGOs cultured for 3 time points (1-3 months) showed a high inter-organoid correlation and ret

SUBMITTER: Verduin M 

PROVIDER: S-EPMC10733660 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature