Nanomolar Protein Thermal Profiling with Modified Cyanine Dyes.

ABSTRACT: Protein properties and interactions have been widely investigated by using external labels. However, the micromolar sensitivity of the current dyes limits their applicability due to the high material consumption and assay cost. In response to this challenge, we synthesized a series of cyanine5 (Cy5) dye-based quencher molecules to develop an external dye technique to probe proteins at the nanomolar protein level in a high-throughput one-step assay format. Several families of Cy5 dye-based quenchers with ring and/or side-chain modifications were designed and synthesized by introducing organic small molecules or peptides. Our results showed that steric hindrance and electrostatic interactions are more important than hydrophobicity in the interaction between the luminescent negatively charged

SUBMITTER: Malakoutikhah M 

PROVIDER: S-EPMC10733900 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature