Simple and Practical DNA Quantification Method for DNA-Encoded Library Synthesis.

ABSTRACT: Over the past three decades, DNA-encoded library (DEL) technologies have become one of the most relevant strategies for hit-finding. Recent advances in synthetic methodologies for DNA-encoded libraries rendered the increased chemical space available, but it is unknown how every variety of chemistry affects DNA's integrity. Available assays to quantify DNA damage are restricted to electrophoresis, ligation efficiency, and mostly qPCR quantification and sequencing, which may contain predisposition and inconsistency. We developed an external standard method through LC-MS analysis to accurately quantify DNA damage throughout the chemical transformations. An assessment was conducted on on-DNA chemical reactions that are frequently employed in DEL synthesis, and these results were compared to tr

SUBMITTER: Ma H 

PROVIDER: S-EPMC10733999 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature