Endosulfine alpha maintains spindle pole integrity by recruiting Aurora A during mitosis.

ABSTRACT:

Background

The maintenance of spindle pole integrity is essential for spindle assembly and chromosome segregation during mitosis. However, the underlying mechanisms governing spindle pole integrity remain unclear.

Methods

ENSA was inhibited by siRNA or MKI-2 treatment and its effect on cell cycle progression, chromosome alignment and microtubule alignment was observed by immunohistochemical staining and western blotting. PP2A-B55α knockdown by siRNA was performed to rescue the phenotype caused by ENSA inhibition. The interaction between ENSA and Aurora A was detected by in situ PLA. Furthermore, orthotopic implantation of 4Tl-luc cancer cells was conducted to confirm the consistency between the in vitro and in vivo relationship of the ENSA-Aurora A interaction.

Results

SUBMITTER: Kim S 

PROVIDER: S-EPMC10734108 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature