Transposons and CRISPR: Rewiring Gene Editing.

ABSTRACT: CRISPR-Cas is driving a gene editing revolution because of its simple reprogramming. However, off-target effects and dependence on the double-strand break repair pathways impose important limitations. Because homology-directed repair acts primarily in actively dividing cells, many of the current gene correction/replacement approaches are restricted to a minority of cell types. Furthermore, current approaches display low efficiency upon insertion of large DNA cargos (e.g., sequences containing multiple gene circuits with tunable functionalities). Recent research has revealed new links between CRISPR-Cas systems and transposons providing new scaffolds that might overcome some of these limitations. Here, we comment on two new transposon-associated RNA-guided mechanisms considering their poten

SUBMITTER: Tenjo-Castano F 

PROVIDER: S-EPMC10734217 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature