Profiling whole-tissue metabolic reprogramming during cutaneous poxvirus infection and clearance.

ABSTRACT:

Importance

Human poxvirus infections have caused significant public health burdens both historically and recently during the unprecedented global Mpox virus outbreak. Although vaccinia virus (VACV) infection of mice is a commonly used model to explore the anti-poxvirus immune response, little is known about the metabolic changes that occur in vivo during infection. We hypothesized that the metabolome of VACV-infected skin would reflect the increased energetic requirements of both virus-infected cells and immune cells recruited to sites of infection. Therefore, we profiled whole VACV-infected skin using untargeted mass spectrometry to define the metabolome during infection, complementing these experiments with flow cytometry and transcriptomics. We identified specific metabol

SUBMITTER: Pei L 

PROVIDER: S-EPMC10734417 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature