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Amino acid substitutions in norovirus VP1 dictate host dissemination via variations in cellular attachment.

ABSTRACT:

Importance

All viruses initiate infection by utilizing receptors to attach to target host cells. These virus-receptor interactions can therefore dictate viral replication and pathogenesis. Understanding the nature of virus-receptor interactions could also be important for the development of novel therapies. Noroviruses are non-enveloped icosahedral viruses of medical importance. They are a common cause of acute gastroenteritis with no approved vaccine or therapy and are a tractable model for studying fundamental virus biology. In this study, we utilized the murine norovirus model system to show that variation in a single amino acid of the major capsid protein alone can affect viral infectivity through improved attachment to suspension cells. Modulating plasma membrane mobility reduced infectivity, suggesting an importance of membrane mobility for receptor recruitment and/or receptor conformation. Furthermore, different substitutions at this site altered viral tissue distribution in a murine model, illustrating how in-host capsid evolution could influence viral infectivity and/or immune evasion.

SUBMITTER: Mills JT

PROVIDER: S-EPMC10734460 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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Amino acid substitutions in norovirus VP1 dictate host dissemination via variations in cellular attachment.

Mills Jake T JT Minogue Susanna C SC Snowden Joseph S JS Arden Wynter K C WKC Rowlands David J DJ Stonehouse Nicola J NJ Wobus Christiane E CE Herod Morgan R MR

Journal of virology 20231130 12

<h4>Importance</h4>All viruses initiate infection by utilizing receptors to attach to target host cells. These virus-receptor interactions can therefore dictate viral replication and pathogenesis. Understanding the nature of virus-receptor interactions could also be important for the development of novel therapies. Noroviruses are non-enveloped icosahedral viruses of medical importance. They are a common cause of acute gastroenteritis with no approved vaccine or therapy and are a tractable model ...[more]

PMID: 38032199

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Dataset Information

Amino acid substitutions in norovirus VP1 dictate host dissemination via variations in cellular attachment.

Importance

Publications

Amino acid substitutions in norovirus VP1 dictate host dissemination via variations in cellular attachment.

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