Juglone, a plant-derived 1,4-naphthoquinone, binds to hydroxylamine oxidoreductase and inhibits the electron transfer to cytochrome c₅₅₄.

ABSTRACT:

Importance

Nitrification, the microbial conversion of ammonia to nitrate via nitrite, plays a pivotal role in the global nitrogen cycle. However, the excessive use of ammonium-based fertilizers in agriculture has disrupted this cycle, leading to groundwater pollution and greenhouse gas emissions. In this study, we have demonstrated the inhibitory effects of plant-derived juglone and related 1,4-naphthoquinones on the nitrification process in Nitrosomonas europaea. Notably, the inhibition mechanism is elucidated in which 1,4-naphthoquinones interact with hydroxylamine oxidoreductase, disrupting the electron transfer to cytochrome c₅₅₄, a physiological electron acceptor. These findings support the notion that phytochemicals can impede nitrification by interfe

SUBMITTER: Akutsu Y 

PROVIDER: S-EPMC10734522 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature