Amplicon-guided isolation and cultivation of previously uncultured microbial species from activated sludge.

ABSTRACT:

Importance

Biological wastewater treatment relies on complex microbial communities that assimilate nutrients and break down pollutants in the wastewater. Knowledge about the physiology and metabolism of bacteria in wastewater treatment plants (WWTPs) may therefore be used to improve the efficacy and economy of wastewater treatment. Our current knowledge is largely based on 16S rRNA gene amplicon profiling, fluorescence in situ hybridization studies, and predictions based on metagenome-assembled genomes. Bacterial isolates are often required to validate genome-based predictions as they allow researchers to analyze a specific species without interference from other bacteria and with simple bulk measurements. Unfortunately, there are currently very few pure cultures representin

SUBMITTER: Verhoeven MD 

PROVIDER: S-EPMC10734543 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature