Single-cell characterization of human GBM reveals regional differences in tumor-infiltrating leukocyte activation.

ABSTRACT: Glioblastoma (GBM) harbors a highly immunosuppressive tumor microenvironment (TME) which influences glioma growth. Major efforts have been undertaken to describe the TME on a single-cell level. However, human data on regional differences within the TME remain scarce. Here, we performed high-depth single-cell RNA sequencing (scRNAseq) on paired biopsies from the tumor center, peripheral infiltration zone and blood of five primary GBM patients. Through analysis of >45,000 cells, we revealed a regionally distinct transcription profile of microglia (MG) and monocyte-derived macrophages (MdMs) and an impaired activation signature in the tumor-peripheral cytotoxic-cell compartment. Comparing tumor-infiltrating CD8⁺ T cells with circulating cells identified CX3CR1^high and CX

SUBMITTER: Schmassmann P 

PROVIDER: S-EPMC10735226 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature