Biodegradation of Petroleum Sludge by Methylobacterium sp. Strain ZASH.

ABSTRACT: A bacterium was isolated from sludge-contaminated soil in a petroleum refinery and tested for its ability to degrade aliphatic hydrocarbon compounds present in petroleum sludge. The isolate was grown on minimal salt media agar supplemented with 1% (w/v) petroleum sludge. The isolate was tentatively identified as Methylobacterium s p. s t rain ZASH based on the partial 16s rDNA molecular phylogeny. The bacterium grew optimally between the temperatures of 30°C and 35°C, pH 7 and 7.5, 0.5% and 1.5% (v/v) Tween 80 as the surfactant, and between 1% and 2% (w/v) peptone as the nitrogen source. The constants derived from the Haldane equation were μmax = 0.039 hr^-1, K_s = 0.385% (w/v) total petroleum hydrocarbons (TPH) or 3,850 mg/L TPH, and K

SUBMITTER: Harumain ZAS 

PROVIDER: S-EPMC10735261 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature