Interferon gamma immunoPET imaging to evaluate response to immune checkpoint inhibitors.

ABSTRACT:

Introduction

We previously developed a ⁸⁹Zr-labeled antibody-based immuno-positron emission tomography (immunoPET) tracer targeting interferon gamma (IFNγ), a cytokine produced predominantly by activated T and natural killer (NK) cells during pathogen clearance, anti-tumor immunity, and various inflammatory and autoimmune conditions. The current study investigated [⁸⁹Zr]Zr-DFO-anti-IFNγ PET as a method to monitor response to immune checkpoint inhibitors (ICIs).

Methods

BALB/c mice bearing CT26 colorectal tumors were treated with combined ICI (anti-cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) and anti-programmed death 1 (PD-1)). The [⁸⁹Zr]Zr-DFO-anti-IFNγ PET tracer, generated with antibody clone AN18, was administered on the day of th

SUBMITTER: Hackett JB 

PROVIDER: S-EPMC10735274 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature