Hierarchical Development of Motile Polarity in Durotactic Cells Just Crossing an Elasticity Boundary.

ABSTRACT: Cellular durotaxis has been extensively studied in the field of mechanobiology. In principle, asymmetric mechanical field of a stiffness gradient generates motile polarity in a cell, which is a driving factor of durotaxis. However, the actual process by which the motile polarity in durotaxis develops is still unclear. In this study, to clarify the details of the kinetics of the development of durotactic polarity, we investigated the dynamics of both cell-shaping and the microscopic turnover of focal adhesions (FAs) for Venus-paxillin-expressing fibroblasts just crossing an elasticity boundary prepared on microelastically patterned gels. The Fourier mode analysis of cell-shaping based on a persistent random deformation model revealed that motile polarity at a cell-body scale was established

SUBMITTER: Kuboki T 

PROVIDER: S-EPMC10739161 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature