Immobilization of azide-functionalized proteins to micro- and nanoparticles directly from cell lysate.

ABSTRACT: Immobilization of proteins and enzymes on solid supports has been utilized in a variety of applications, from improved protein stability on supported catalysts in industrial processes to fabrication of biosensors, biochips, and microdevices. A critical requirement for these applications is facile yet stable covalent conjugation between the immobilized and fully active protein and the solid support to produce stable, highly bio-active conjugates. Here, we report functionalization of solid surfaces (gold nanoparticles and magnetic beads) with bio-active proteins using site-specific and biorthogonal labeling and azide-alkyne cycloaddition, a click chemistry. Specifically, we recombinantly express and selectively label calcium-dependent proteins, calmodulin and calcineurin, and cAMP-dependent

SUBMITTER: Saini G 

PROVIDER: S-EPMC10739308 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature