Copper-induced diurnal hepatic toxicity is associated with Cry2 and Per1 in mice.

ABSTRACT:

Background

This study aimed to investigate diurnal variations in copper-induced hepatic toxicity and the molecular mechanisms underlying this chronotoxicity.

Methods

Male C57BL/6J mice were intraperitoneally injected with copper chloride (CuCl₂) at zeitgeber time 2 (ZT2) or 14 (ZT14), twice per week for 5 or 8 weeks. Seventy-two hours after the final CuCl₂ injection, the mice were euthanized, and plasma samples were collected. The livers and kidneys were collected and weighed. In vitro experiments were performed to assess cell viability and fluctuations in clock gene expression levels in Hepa1-6 cells after CuCl₂ treatment. We examined copper homeostasis- and apoptosis-related genes under clock genes overexpression.

Results

Repeated Cu

SUBMITTER: Tominaga S 

PROVIDER: S-EPMC10739358 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature