Solid-Phase Synthesis of Caged Luminescent Peptides via Side Chain Anchoring.

ABSTRACT: The synthesis of caged luminescent peptide substrates remains challenging, especially when libraries of the substrates are required. Most currently available synthetic methods rely on a solution-phase approach, which is less suited for parallel synthesis purposes. We herein present a solid-phase peptide synthesis (SPPS) method for the synthesis of caged aminoluciferin peptides via side chain anchoring of the P₁ residue. After the synthesis of a preliminary test library consisting of 40 compounds, the synthetic method was validated and optimized for up to >100 g of resin. Subsequently, two separate larger peptide libraries were synthesized either having a P₁ = lysine or arginine residue containing in total 719 novel peptide substrates. The use of a more stable caged ni

SUBMITTER: Sondag D 

PROVIDER: S-EPMC10739589 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature