Noninvasive measurement of ¹³Carbon turnover for evaluation of porcine renal grafts during ex vivo machine perfusion.

ABSTRACT:

Background

Kidney transplantation suffers from a shortage of donor organs. Despite this, a lot of grafts are discarded due to inadequate quality. As many kidneys are afflicted by transient filtration failure early after preservation, classical renal function tests are not applicable to differentiate between prospective recovery or continuing deficit of renal function.

Methods

Using normothermic machine perfusion as a platform for pre-implantation evaluation of the graft, we present a novel evaluative approach based on the metabolic turnover of ¹³C-acetate during isolated perfusion. After injection of the tracer, ¹³CO₂ as a metabolic end-product can be quantified by high-precision laser-based spectroscopy in the gas outflow of the oxygenator.

SUBMITTER: Minor T 

PROVIDER: S-EPMC10739878 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature