Efficient and reproducible generation of human induced pluripotent stem cell-derived expandable liver organoids for disease modeling.

ABSTRACT: Genetic liver disease modeling is difficult because it is challenging to access patient tissue samples and to develop practical and relevant model systems. Previously, we developed novel proliferative and functional liver organoids from pluripotent stem cells; however, the protocol requires improvement for standardization and reproducible mass production. Here, we improved the method such that it is suitable for scalable expansion and relatively homogenous production, resulting in an efficient and reproducible process. Moreover, three medium components critical for long-term expansion were defined. Detailed transcriptome analysis revealed that fibroblast growth factor signaling, the essential pathway for hepatocyte proliferation during liver regeneration, was mainly enriched in proliferati

SUBMITTER: Mun SJ 

PROVIDER: S-EPMC10739970 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature