Luciferase Calibrants Enable Absolute Quantitation of Bioluminescence Power.

ABSTRACT: Bioluminescence emitted from a luciferase-catalyzed oxidation of luciferin has been broadly utilized to report on biological events, predominantly through relative changes in the light output. Recent advances in protein engineering and synthetic chemistry have yielded bioluminescent systems with markedly improved brightness and bioavailability. These developments have enabled not only the detection of biological events at far lower expression levels but also new opportunities utilizing bioluminescence to power photochemistry in cells. Regardless of the application, bioluminescence analyses have leaned heavily on the use of luminometers to measure the light output of a system. Current luminometers report the light output of a sample in relative units, limiting the ability to compare data be

SUBMITTER: Klein MA 

PROVIDER: S-EPMC10740115 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature