Dataset Information

Genetic influence on urinary vitamin D binding protein excretion and serum levels: a focus on rs4588 C>A polymorphism in the GC gene.

ABSTRACT:

Introduction

Vitamin D binding protein (VDBP) plays a crucial role in vitamin D transport and metabolism. The rs4588-A polymorphism of the GC gene, encoding VDBP, has been associated with altered serum VDBP and 25-hydroxyvitamin D (25OHD) levels. However, the mechanisms underlying these effects remain unclear. We aimed to investigate the relationship between urinary VDBP excretion and serum VDBP and 25OHD levels in individuals with and without the rs4588-A allele.

Methods

A cross-sectional study was conducted on 109 children (mean age: 11.96 years) to explore the impact of rs4588-A on vitamin D metabolism and urinary VDBP excretion. Biochemical analyses determined serum 25OHD and VDBP levels, and urinary VDBP-to-creatinine ratio (u-VDBP/Cr). Genotyping for rs4588 SNP was performed using LightSNiP assay. Statistical analyses included correlation, linear regression, and comparison between allele groups.

Results

Participants carrying the rs4588-A allele exhibited lower serum 25OHD levels compared to non-carriers (median (IQR): 11.85 (3.5) vs. 12.86 (4.9), p = 0.023). However, no statistically significant differences were observed in serum VDBP levels (126.34 ± 59.3 in rs4588-A vs. 136.49 ± 51.3 in non-rs4588-A, p = 0.141) or in u-VDBP/Cr (median (IQR): 0.4 (0.35) in rs4588-A vs. 0.386 (0.43) in non-rs4588-A, p = 0.189) between the two allele groups. A significant inverse correlation between u-VDBP/Cr and serum VDBP levels was found only in rs4588-A carriers (r = -0.367, p = 0.024). No such correlation was observed in non-carriers or the entire cohort. A linear regression analysis confirmed the impact of u-VDBP/Cr on serum VDBP levels in rs4588-A carriers (B = -0.269, t = -2.185, p = 0.035).

Conclusion

Individuals with the rs4588-A allele in the GC gene had lower serum 25OHD levels. An inverse correlation between urinary VDBP excretion and serum VDBP levels was observed, suggesting a partial role of the renal pathway in altered serum VDBP and 25OHD levels linked to the rs4588-A allele.

SUBMITTER: Dogan D

PROVIDER: S-EPMC10740204 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Publications

Genetic influence on urinary vitamin D binding protein excretion and serum levels: a focus on rs4588 C>A polymorphism in the GC gene.

Doğan Durmus D Özcan Eda Gül EG Çakır Dilek Ülker DÜ Sılan Fatma F

Frontiers in endocrinology 20231207

<h4>Introduction</h4>Vitamin D binding protein (VDBP) plays a crucial role in vitamin D transport and metabolism. The rs4588-A polymorphism of the GC gene, encoding VDBP, has been associated with altered serum VDBP and 25-hydroxyvitamin D (25OHD) levels. However, the mechanisms underlying these effects remain unclear. We aimed to investigate the relationship between urinary VDBP excretion and serum VDBP and 25OHD levels in individuals with and without the rs4588-A allele.<h4>Methods</h4>A cross- ...[more]

PMID: 38144557

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Project description:Abstract Objectives Dietary advanced glycation end products (AGEs) are a group of compounds formed by the Maillard reaction. Carboxymethyl-lysine (CML), a marker of AGEs in foods, may contribute to cardiometabolic diseases. However, CML health effects and its absorption and excretion in healthy individuals remained controversial. For this reason, the aim of this study was to evaluate serum and urinary excretion CML levels in healthy adults after a short-term diet high in CML. Methods A randomized clinical trial was conducted in 30 healthy adults (18–35 years old). After signing the informed consent, subjects were randomly assigned to a low or a high CML diet. All participant followed a diet with low CML content for seven days (washing period) before the 5-days intervention. During the 5 days participants received all the foods according to their group. Blood and 24-hour urine samples were collected before and after the intervention to evaluate serum and urinary CML levels. CML measurement in foods, serum, and urine was carried out by HPLC-ESI-ITMS/MS. Comparison between groups was carried out by a t-test. The research was approved by the Institutional Review Board and the clinical trial registration number NCT03208946. Results Biochemical and anthropometric variables were similar between groups (P > 0.05). The average consumption of CML was 1.93 ± 1.47 mg/day for the low CML diet and 5.03 ± 2.22 mg/day for the high CML diet, whereas caloric, protein and fat intake were similar. Serum levels decreased significantly in both groups (low diet: basal 488.1 ± 135.4 μg/L, final 383.2 ± 78.6 μg/L (P = 0.007)) (high diet: basal 463.5 ± 90.3 μg/L, final 390.3 ± 99.1 μg/L (P = 0.02)). No significant difference was found when comparing the final CML levels between groups (P = 0.47). The excretion of CML in urine was adjusted by creatinine (mg) and the high CML group had a greater excretion (4.5 ± 8.0 ug CML/mg) when compared to the low CML group (−1.5 ± 7.2 ug CML/mg) (P = 0.04). Conclusions A high CML diet increases urinary CML excretion levels in healthy adults when compared to a low CML diet. There were no significant differences in serum CML levels between both groups. However, a greater decrease was observed in the subjects who followed a diet with a low content of AGEs. Funding Sources University of Guanajuato. CONACYT.

Dataset Information

Genetic influence on urinary vitamin D binding protein excretion and serum levels: a focus on rs4588 C>A polymorphism in the GC gene.

Introduction

Methods

Results

Conclusion

Publications

Genetic influence on urinary vitamin D binding protein excretion and serum levels: a focus on rs4588 C>A polymorphism in the GC gene.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Genetic influence on urinary vitamin D binding protein excretion and serum levels: a focus on rs4588 C>A polymorphism in the GC gene.

Introduction

Methods

Results

Conclusion

Publications

Genetic influence on urinary vitamin D binding protein excretion and serum levels: a focus on rs4588 C&gt;A polymorphism in the GC gene.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Genetic influence on urinary vitamin D binding protein excretion and serum levels: a focus on rs4588 C>A polymorphism in the GC gene.