Effect of miR-21 in mesenchymal stem cells-derived extracellular vesicles behavior.

ABSTRACT:

Background

A challenging new branch of research related to aging-associated diseases is the identification of miRNAs capable of modulating the senescence-associated secretory phenotype (SASP) which characterizes senescent cells and contributes to driving inflammation.

Methods

Mesenchymal stem cells (MSC) from human umbilical cord stroma were stable modified using lentivirus transduction to inhibit miR-21-5p and shotgun proteomic analysis was performed in the MSC-derived extracellular vesicles (EV) to check the effect of miR-21 inhibition in their protein cargo. Besides, we studied the paracrine effect of those modified extracellular vesicles and also their effect on SASP.

Results

Syndecan-1 (SDC1) was the most decreased protein in MSC-miR21^--derived EV, and

SUBMITTER: Morente-Lopez M 

PROVIDER: S-EPMC10740217 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature