Dataset Information

Covariation between microeukaryotes and bacteria associated with Planorbidae snails.

ABSTRACT:

Background

Microbial communities associated with macroorganisms might affect host physiology and homeostasis. Bacteria are well studied in this context, but the diversity of microeukaryotes, as well as covariations with bacterial communities, remains almost unknown.

Methods

To study microeukaryotic communities associated with Planorbidae snails, we developed a blocking primer to reduce amplification of host DNA during metabarcoding analyses. Analyses of alpha and beta diversities were computed to describe microeukaryotes and bacteria using metabarcoding of 18S and 16S rRNA genes, respectively.

Results

Only three phyla (Amoebozoa, Opisthokonta and Alveolata) were dominant for microeukaryotes. Bacteria were more diverse with five dominant phyla (Proteobacteria, Bacteroidetes, Tenericutes, Planctomycetes and Actinobacteria). The composition of microeukaryotes and bacteria were correlated for the Biomphalaria glabrata species, but not for Planorbarius metidjensis. Network analysis highlighted clusters of covarying taxa. Among them, several links might reflect top-down control of bacterial populations by microeukaryotes, but also possible competition between microeukaryotes having opposite distributions (Lobosa and Ichthyosporea). The role of these taxa remains unknown, but we believe that the blocking primer developed herein offers new possibilities to study the hidden diversity of microeukaryotes within snail microbiota, and to shed light on their underestimated interactions with bacteria and hosts.

SUBMITTER: Clerissi C

PROVIDER: S-EPMC10740603 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Publications

Covariation between microeukaryotes and bacteria associated with Planorbidae snails.

Clerissi Camille C Huot Camille C Portet Anaïs A Gourbal Benjamin B Toulza Eve E

PeerJ 20231219

<h4>Background</h4>Microbial communities associated with macroorganisms might affect host physiology and homeostasis. Bacteria are well studied in this context, but the diversity of microeukaryotes, as well as covariations with bacterial communities, remains almost unknown.<h4>Methods</h4>To study microeukaryotic communities associated with Planorbidae snails, we developed a blocking primer to reduce amplification of host DNA during metabarcoding analyses. Analyses of alpha and beta diversities ...[more]

PMID: 38144201

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Dataset Information

Covariation between microeukaryotes and bacteria associated with Planorbidae snails.

Background

Methods

Results

Publications

Covariation between microeukaryotes and bacteria associated with Planorbidae snails.

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