Multi-moded high-index contrast optical waveguide for super-contrast high-resolution label-free microscopy.

ABSTRACT: The article elucidates the physical mechanism behind the generation of superior-contrast and high-resolution label-free images using an optical waveguide. Imaging is realized by employing a high index contrast multi-moded waveguide as a partially coherent light source. The modes provide near-field illumination of unlabeled samples, thereby repositioning the higher spatial frequencies of the sample into the far-field. These modes coherently scatter off the sample with different phases and are engineered to have random spatial distributions within the integration time of the camera. This mitigates the coherent speckle noise and enhances the contrast (2-10) × as opposed to other imaging techniques. Besides, the coherent scattering of the different modes gives rise to fluctuations in intensity

SUBMITTER: Jayakumar N 

PROVIDER: S-EPMC10741054 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature