Heterologous Production of Isopropanol Using Metabolically Engineered Acetobacterium woodii Strains.

ABSTRACT: The depletion of fossil fuel resources and the CO₂ emissions coupled with petroleum-based industrial processes present a relevant issue for the whole of society. An alternative to the fossil-based production of chemicals is microbial fermentation using acetogens. Acetogenic bacteria are able to metabolize CO or CO₂ (+H₂) via the Wood-Ljungdahl pathway. As isopropanol is widely used in a variety of industrial branches, it is advantageous to find a fossil-independent production process. In this study, Acetobacterium woodii was employed to produce isopropanol via plasmid-based expression of the enzymes thiolase A, CoA-transferase, acetoacetate decarboxylase and secondary alcohol dehydrogenase. An examination of the enzymes originating from different org

SUBMITTER: Hofele F 

PROVIDER: S-EPMC10741115 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature