Targeting Microglia/Macrophages Notch1 Protects Neurons from Pyroptosis in Ischemic Stroke.

ABSTRACT:

Background and aims

The immune-inflammatory cascade and pyroptosis play an important role in the pathogenesis of cerebral ischemia-reperfusion injury (CIRI). The maintenance of immune homeostasis is inextricably linked to the Notch signaling pathway, but whether myeloid Notch1 affects microglia polarization as well as neuronal pyroptosis in CIRI is not fully understood. This study was designed to clarify the role of myeloid Notch1 in CIRI, providing new therapeutic strategies for ischemic stroke.

Methods and results

Myeloid-specific Notch1 knockout (Notch1^M-KO) mice and the floxed Notch1 (Notch1^FL/FL) mice were subjected to middle cerebral artery occlusion (MCAO). After 3 days of CIRI, we evaluated the neurological deficit score and cerebral infarction

SUBMITTER: Chen R 

PROVIDER: S-EPMC10741505 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature