A Novel Aptamer Biosensor Based on a Localized Surface Plasmon Resonance Sensing Chip for High-Sensitivity and Rapid Enrofloxacin Detection.

ABSTRACT: Enrofloxacin, a fluoroquinolone widely used in animal husbandry, presents environmental and human health hazards due to its stability and incomplete hydrolysis leading to residue accumulation. To address this concern, a highly sensitive aptamer biosensor utilizing a localized surface plasmon resonance (LSPR) sensing chip and microfluidic technology was developed for rapid enrofloxacin residue detection. AuNPs were prepared by the seed method and the AuNPs-Apt complexes were immobilized on the chip by the sulfhydryl groups modified on the end of the aptamer. The properties and morphologies of the sensing chip and AuNPs-Apt complexes were characterized by Fourier transform infrared spectroscopy (FTIR), UV-Vis spectrophotometer, and scanning electron microscope (SEM), respectively. The sensin

SUBMITTER: Wang P 

PROVIDER: S-EPMC10741520 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature