Design of a Facile Antifouling Sensor Based on the Synergy between an Antibody and Phase-Transited BSA.

ABSTRACT: Nonspecific adsorption has always been a critical challenge for sensor detection; thus, an efficient and facile approach for fabricating antifouling sensors is highly desirable. Here, we developed an antifouling coating on sensor surfaces, conveniently made with a simple drip of phase-transited BSA (PTB) followed by a modification with a peanut allergen antibody, which unexpectedly provides synergistic antifouling properties in sensors. Atomic force microscopy and scanning electron microscopy were used to evaluate the surface evenness. Optimizations in terms of PTB modification time and concentrations were performed using surface plasmon resonance by measuring protein resistance capabilities. Compared to bare Au surfaces, the PTB-modified surfaces exhibited low adsorption against BSA (<10

SUBMITTER: Wang S 

PROVIDER: S-EPMC10741890 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature