Overexpression of KvCHX Enhances Salt Tolerance in Arabidopsis thaliana Seedlings.

ABSTRACT: The CHX (cation/H⁺ exchanger) family plays an important role in the transmembrane transport of cation/H⁺ in plants. The aim of this study was to identify and functionally analyze the KvCHX gene in the halophyte Kosteletzkya virginica to investigate its role in regulating the K⁺/Na⁺ ratio under salinity tolerance. Based on a partial gene sequence of EST from K. virginica, the full-length DNA sequence of the KvCHX gene was obtained using genome walking technology. Structural analysis and phylogenetic relationship analysis showed that the KvCHX gene was closely related to the AtCHX17 gene. The KvCHX overexpression vector was successfully constructed and transformed into Arabidopsis via floral dipping.

SUBMITTER: Guo Y 

PROVIDER: S-EPMC10741925 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature