Dataset Information

Endogenously Produced Jasmonates Affect Leaf Growth and Improve Osmotic Stress Tolerance in Emmer Wheat.

ABSTRACT: In light of recent climate change, with its rising temperatures and precipitation changes, we are facing the need to increase the valuable crop's tolerance against unfavorable environmental conditions. Emmer wheat is a cereal crop with high nutritional value. We investigated the possibility of improving the stress tolerance of emmer wheat by activating the synthesis of the stress hormone jasmonate by overexpressing two genes of the jasmonate biosynthetic pathway from Arabidopsis thaliana, ALLENE OXIDE SYNTHASE (AtAOS) and OXOPHYTODIENOATE REDUCTASE 3 (AtOPR3). Analyses of jasmonates in intact and mechanically wounded leaves of non-transgenic and transgenic plants showed that the overexpression of each of the two genes resulted in increased wounding-induced levels of jasmonic acid and jasmonate-isoleucine. Against all expectations, the overexpression of AtAOS, encoding a chloroplast-localized enzyme, does not lead to an increased level of the chloroplast-formed 12-oxo-phytodienoic acid (OPDA), suggesting an effective conversion of OPDA to downstream products in wounded emmer wheat leaves. Transgenic plants overexpressing AtAOS or AtOPR3 with increased jasmonate levels show a similar phenotype, manifested by shortening of the first and second leaves and elongation of the fourth leaf, as well as increased tolerance to osmotic stress induced by the presence of the polyethylene glycol (PEG) 6000.

SUBMITTER: Pigolev AV

PROVIDER: S-EPMC10742046 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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Endogenously Produced Jasmonates Affect Leaf Growth and Improve Osmotic Stress Tolerance in Emmer Wheat.

Pigolev Alexey V AV Miroshnichenko Dmitry N DN Dolgov Sergey V SV Alekseeva Valeria V VV Pushin Alexander S AS Degtyaryova Vlada I VI Klementyeva Anna A Gorbach Daria D Leonova Tatiana T Basnet Aditi A Frolov Andrej A AA Savchenko Tatyana V TV

Biomolecules 20231212 12

In light of recent climate change, with its rising temperatures and precipitation changes, we are facing the need to increase the valuable crop's tolerance against unfavorable environmental conditions. Emmer wheat is a cereal crop with high nutritional value. We investigated the possibility of improving the stress tolerance of emmer wheat by activating the synthesis of the stress hormone jasmonate by overexpressing two genes of the jasmonate biosynthetic pathway from <i>Arabidopsis thaliana</i>, ...[more]

PMID: 38136646

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Project description:High temperatures pose a major threat to wheat productivity and necessitate the development of new cultivars that are resilient to future heat stress. Wild emmer (Triticum turgidum L. ssp. dicoccoides), which is a direct progenitor of domesticated durum wheat (Triticum turgidum L. ssp. durum) and contributor to the A and B genome of bread wheat (Triticum aestivum), offers a valuable genetic reservoir for developing climate-resilient wheat. However, the morphology of wild emmer is different from that of durum and bread wheat, in particular, the spikelets are fragile and naturally fall off, making it difficult to study its agronomic traits. In this study, we created nine backcrossed families between the popular durum wheat cultivar 'Miki 3' and nine wild emmer accessions collected from northern and southern lineages of this species. The objective was to investigate the intraspecific genetic variation in wild emmer and identify traits associated with heat stress tolerance. We evaluated these nine families under multi-environments ranging from optimum to severe heat stress conditions in Japan and Sudan and measured important agronomic traits. The result showed that two families, developed from accessions of both northern and southern lineages exhibited high harvest index, elevated chlorophyll content, and reduced canopy temperature under heat stress. Additionally, one family developed from an accession of the southern lineage displayed high biomass, harvest index, and seed number under heat-stress conditions. These three families produced high heat tolerant lines with unique introgressed segments from their wild emmer parents on chromosomes 1A, 2B, 5B, 6B, and 7B, which may be linked to heat resilience. From these results, we were able to identify significant intraspecific diversity between the wild emmer accessions in terms of heat stress tolerance. However, no significant tendency between the northern and southern lineages of wild emmer has been identified. These findings emphasize the need to harness not only the interspecific but also the intraspecific genetic variation of wild emmer diversity to uncover valuable genes for heat stress tolerance in wheat breeding programs.

Dataset Information

Endogenously Produced Jasmonates Affect Leaf Growth and Improve Osmotic Stress Tolerance in Emmer Wheat.

Publications

Endogenously Produced Jasmonates Affect Leaf Growth and Improve Osmotic Stress Tolerance in Emmer Wheat.

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