Statistical Study of Low-Intensity Single-Molecule Recognition Events Using DeepTip^TM Probes: Application to the Pru p 3-Phytosphingosine System.

ABSTRACT: The interaction between the plant lipid transfer protein Pru p 3 and phytosphingosine was assessed using an atomic force microscope. Phytosphingosine was covalently immobilized on DeepTip^TM probes and Pru p 3 on MicroDeck^TM functionalized substrates. Single-molecular interaction events between both molecules were retrieved and classified and the distribution for each one of the identified types was calculated. A success rate of over 70% was found by comparing the number of specific Pru p 3-phytosphingosine interaction events with the total number of recorded curves. The analysis of the distribution established among the various types of curves was further pursued to distinguish between those curves that can mainly be used for assessing the recognition between phytosph

SUBMITTER: Daza R 

PROVIDER: S-EPMC10742132 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature