Amplification of Signal on Cell Surfaces in Molecular Cascades.

ABSTRACT: We can formulate mixtures of oligonucleotide-antibody conjugates to act as molecular cascade-based automata that analyze pairs of cell surface markers (CD markers) on individual cells in a manner consistent with the implementation of Boolean logic-for example, by producing a fluorescent label only if two markers are present. While traditional methods to characterize cells are based on transducing signals from individual cell surface markers, these cascades can be used to combine into a single signal the presence of two or even more CDs. In our original design, oligonucleotide components irreversibly flowed from one antibody to another, driven by increased hybridizations, leading to the magnitude of the final signal on each cell being determined by the surface marker that was the least abun

SUBMITTER: Rudchenko S 

PROVIDER: S-EPMC10742280 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature