CRISPR screening in human trophoblast stem cells reveals both shared and distinct aspects of human and mouse placental development.

ABSTRACT: The placenta serves as the interface between the mother and fetus, facilitating the exchange of gases and nutrients between their separate blood circulation systems. Trophoblasts in the placenta play a central role in this process. Our current understanding of mammalian trophoblast development relies largely on mouse models. However, given the diversification of mammalian placentas, findings from the mouse placenta cannot be readily extrapolated to other mammalian species, including humans. To fill this knowledge gap, we performed CRISPR knockout screening in human trophoblast stem cells (hTSCs). We targeted genes essential for mouse placental development and identified more than 100 genes as critical regulators in both human hTSCs and mouse placentas. Among them, we further characterized

SUBMITTER: Shimizu T 

PROVIDER: S-EPMC10742386 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature