Comparison of Yersinia enterocolitica DNA Methylation at Ambient and Host Temperatures.

ABSTRACT: Pathogenic bacteria recognize environmental cues to vary gene expression for host adaptation. Moving from ambient to host temperature, Yersinia enterocolitica responds by immediately repressing flagella synthesis and inducing the virulence plasmid (pYV)-encoded type III secretion system. In contrast, shifting from host to ambient temperature requires 2.5 generations to restore motility, suggesting a link to the cell cycle. We hypothesized that differential DNA methylation contributes to temperature-regulated gene expression. We tested this hypothesis by comparing single-molecule real-time (SMRT) sequencing of Y. enterocolitica DNA from cells growing exponentially at 22 °C and 37 °C. The inter-pulse duration ratio rather than the traditional QV scoring was the kinetic metric t

SUBMITTER: Van Hofwegen DJ 

PROVIDER: S-EPMC10742451 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature