The Genome of the Yellow Mealworm, Tenebrio molitor: It's Bigger Than You Think.

ABSTRACT:

Background

Insects are a sustainable source of protein for human food and animal feed. We present a genome assembly, CRISPR gene editing, and life stage-specific transcriptomes for the yellow mealworm, Tenebrio molitor, one of the most intensively farmed insects worldwide.

Methods

Long and short reads and long-range data were obtained from a T. molitor male pupa. Sequencing transcripts from 12 T. molitor life stages resulted in 279 million reads for gene prediction and genetic engineering. A unique plasmid delivery system containing guide RNAs targeting the eye color gene vermilion flanking the muscle actin gene promoter and EGFP marker was used in CRISPR/Cas9 transformation.

Results

The assembly is approximately 53% of the genome si

SUBMITTER: Oppert B 

PROVIDER: S-EPMC10742464 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature