Comparative Application of Terminal Restriction Fragment Analysis Tools to Large-Scale Genomic Assays.

ABSTRACT: The analysis of telomere length is an important component of many studies aiming to characterize the role of telomere maintenance mechanisms in cellular lifespan, disease, or in general chromosome protection and DNA replication pathways. Several powerful methods to accurately measure the telomere length from Southern blots have been developed, but their utility for large-scale genomic studies has not been previously evaluated. Here, we performed a comparative analysis of two recently developed programs, TeloTool and WALTER, for the extraction of mean telomere length values from Southern blots. Using both software packages, we measured the telomere length in two extensive experimental datasets for the model plant Arabidopsis thaliana, consisting of 537 natural accessions and 65 T-DNA

SUBMITTER: Abdulkina LR 

PROVIDER: S-EPMC10742804 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature