Efficient delivery of small interfering RNA for inhibition of IL-12p40 expression in vivo.

ABSTRACT:

Background

RNA interference is an evolutionary conserved immune response mechanism that can be used as a tool to provide novel insights into gene function and structure. The ability to efficiently deliver small interfering RNA to modulate gene expression in vivo may provide new therapeutic approaches to currently intractable diseases.

Methods

In vitro, siRNA targeting IL-12p40 was delivered to the murine macrophage cell line (J774A.1) encapsulated in a liposome with an IL-12 inducing agent (LPS/IFN-γ) over a number of time points. Controls included a variety of non-target specific siRNA reagents. Supernatants were analyzed for cytokine production while the cells were removed for mRNA profiling. In vivo, siRNA-targeting IL-12p40 was delivered to the murin

SUBMITTER: Flynn MA 

PROVIDER: S-EPMC1074346 | biostudies-literature | 2004

REPOSITORIES: biostudies-literature