Untangling Irregular Actin Cytoskeleton Architectures in Tomograms of the Cell with Struwwel Tracer.

ABSTRACT: In this work, we established, validated, and optimized a novel computational framework for tracing arbitrarily oriented actin filaments in cryo-electron tomography maps. Our approach was designed for highly complex intracellular architectures in which a long-range cytoskeleton network extends throughout the cell bodies and protrusions. The irregular organization of the actin network, as well as cryo-electron-tomography-specific noise, missing wedge artifacts, and map dimensions call for a specialized implementation that is both robust and efficient. Our proposed solution, Struwwel Tracer, accumulates densities along paths of a specific length in various directions, starting from locally determined seed points. The highest-density paths originating from the seed points form short lin

SUBMITTER: Sazzed S 

PROVIDER: S-EPMC10743648 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature