High-Resolution Structure and Internal Mobility of a Plant 40S Ribosomal Subunit.

ABSTRACT: Ribosome is a major part of the protein synthesis machinery, and analysis of its structure is of paramount importance. However, the structure of ribosomes from only a limited number of organisms has been resolved to date; it especially concerns plant ribosomes and ribosomal subunits. Here, we report a high-resolution cryo-electron microscopy reconstruction of the small subunit of the Triticum aestivum (common wheat) cytoplasmic ribosome. A detailed atomic model was built that includes the majority of the rRNA and some of the protein modifications. The analysis of the obtained data revealed structural peculiarities of the 40S subunit in the monocot plant ribosome. We applied the 3D Flexible Refinement approach to analyze the internal mobility of the 40S subunit and succeeded in decom

SUBMITTER: Kravchenko OV 

PROVIDER: S-EPMC10743738 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature