Mismatch Repair Protein Msh6^Tt Is Necessary for Nuclear Division and Gametogenesis in Tetrahymena thermophila.

ABSTRACT: DNA mismatch repair (MMR) improves replication accuracy by up to three orders of magnitude. The MutS protein in E. coli or its eukaryotic homolog, the MutSα (Msh2-Msh6) complex, recognizes base mismatches and initiates the mismatch repair mechanism. Msh6 is an essential protein for assembling the heterodimeric complex. However, the function of the Msh6 subunit remains elusive. Tetrahymena undergoes multiple DNA replication and nuclear division processes, including mitosis, amitosis, and meiosis. Here, we found that Msh6^Tt localized in the macronucleus (MAC) and the micronucleus (MIC) during the vegetative growth stage and starvation. During the conjugation stage, Msh6^Tt only localized in MICs and newly developing MACs. MSH6^Tt knockout l

SUBMITTER: Wang L 

PROVIDER: S-EPMC10743813 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature